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Characterization of the dystrophic muscle by ²³Na NMR and ¹H NMR T₂ spectrum

Abstract : The aim of the thesis is to investigate the sensitivity of novel NMR outcome measures (OM) aiming to quantify pathological changes in the dystrophic muscle. Muscular dystrophy (MD) refers to a heterogeneous group of diseases with progressive muscle wasting and associated weakness characterized by variable degrees of necrosis, regeneration, ionic homeostasis disturbances, chronic inflammation, and, ultimately, resulting in the replacement of muscles by fibro-fatty tissue. My focus was on the evaluation of ²³Na NMR and advanced ¹H transverse relaxation time (T₂) techniques as early, sensitive OM. ²³Na NMR measures the tightly controlled sodium concentrations and distribution in skeletal muscle tissue. This biophysical information can be used to assess ion homeostasis and cell integrity. However, ²³Na NMR suffers from a low sensitivity and in vivo concentration compared to ¹H. Alterations in the muscle ¹H T₂, commonly interpreted as an indicator of disease activity, are linked to a variety of non-specific events like oedema, inflammation, or necrosis that precede the actual muscle replacement by fat. Protocols including different ²³Na NMR and ¹H T₂ methods were implemented to evaluate healthy and dystrophic skeletal muscle tissues of animal models and patients. This work provides evidence that ²³Na NMR could offer a sensitive outcome measure able to monitor specific alteration of the dystrophic muscle at a very early stage.
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Submitted on : Wednesday, July 18, 2018 - 9:55:18 AM
Last modification on : Wednesday, October 14, 2020 - 4:10:31 AM
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  • HAL Id : tel-01842257, version 1



Teresa Gerhalter. Characterization of the dystrophic muscle by ²³Na NMR and ¹H NMR T₂ spectrum. Medical Physics []. Université Paris Saclay (COmUE); Freie Universität (Berlin), 2018. English. ⟨NNT : 2018SACLS219⟩. ⟨tel-01842257⟩



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