Skip to Main content Skip to Navigation
Theses

Auto-assemblage de protéines pour la bioélectronique : étude du tranport de charges dans les fibres amyloïdes

Abstract : Amyloid fibers are very promising biomaterials for bioelectronics, especially for interfacing with biological systems. These self-assembled proteins fibers are easy to synthetize, to tune and to functionalize. Their physical properties such as stability and mechanical strength are noticeable. We studied charge transport processes in HET-s(218-289), the only amyloid fibers we know the atomic structure. The samples were characterized as “dried” films by electrical measurement and electrochemistry. The influence of several parameters such as temperature, humidity or light was investigated. We demonstrated that the fiber organization allows intrinsic charge transport mechanisms in which water plays a crucial role. Furthermore, the dominant charge carriers would be protons. Molecular dynamic simulation and neutron diffusion experiments run in parallel show strong water-fibers interactions. In particular, H-bonded water wires can be formed along the fibers and support proton transport according to a Grotthuss-like mechanism. Proton production would result from electrochemical reactions, especially from water electrolysis. Therefore a catalytic current is detected when the bias exceeds a certain threshold. In addition, two photoelectric phenomena were observed when the fibers are irradiated with near UV light (200-400nm). The first one is a photocurrent probably due to water photo-splitting. The other occurs specifically at 280nm wavelength and in the presence of molecular oxygen. It leads to a decrease of the sample conductivity. This likely results from chemical reaction(s) between triplet-state tryptophan and oxygen that consumes protons.
Complete list of metadatas

Cited literature [248 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01835473
Contributor : Abes Star :  Contact
Submitted on : Wednesday, July 11, 2018 - 1:59:06 PM
Last modification on : Friday, July 10, 2020 - 7:58:55 AM
Long-term archiving on: : Saturday, October 13, 2018 - 1:55:07 AM

File

RONGIER_2018_diffusion.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01835473, version 1

Collections

Citation

Anaëlle Rongier. Auto-assemblage de protéines pour la bioélectronique : étude du tranport de charges dans les fibres amyloïdes. Biophysique [physics.bio-ph]. Université Grenoble Alpes, 2018. Français. ⟨NNT : 2018GREAY006⟩. ⟨tel-01835473⟩

Share

Metrics

Record views

294

Files downloads

397