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Caractérisation fonctionnelle de petites protéines sécrétées chez les champignons lignolytiques

Abstract : During the last decades, the enzymatic systems involved in wood degradation have been intensively studied in fungi. This has led to functional and biochemical characterization of the main extracellular enzymes that are involved in the process. However, other systems associated to the degradation mechanisms have been poorly studied. In particular, the detoxification and stress response pathways allowing the fungus to grow in and resist the toxic conditions that are associated to the degradative process are still unknown. This stress is mostly due to the presence of radicals and extractives. Extractives are putative toxic compounds produced as secondary metabolites in tree to enhance wood durability against biotic and abiotic attacks. A transcriptomic analysis performed in the laboratory highlighted the up-regulation of genes coding for small secreted proteins (SSP) in Phanerochaete chrysosporium in presence of oak extractives. The functions of these SSP are unknown in lignolytic fungi. My PhD project was focused on the characterization of one of these SSP (namely SSP1) of P. chrysosporium. The biochemical data revealed atypical features for SSP1. Indeed, it is able to form fibrilar structure, thanks to an alanine-rich and glycine-rich C-terminal domain. Moreover, we have shown that this protein exhibits β-glucuronidase activity in vitro which is dependent on its oligomerization state. Physiological data were obtained thanks to the obtention of SSP knock-out mutants in Podospora anserina. These mutants have growth defect in oxidizing stress condition and in presence of cell wall-disruptive compounds. Finally, the in silico analysis of SSP1 orthologues revealed the presence of this gene in genomes of saprophytic, ectomycorrhizal or pathogenic fungi, suggesting an indirect role of this protein in wood degradation processes, probably linked to the associated stress
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Submitted on : Tuesday, July 10, 2018 - 6:16:08 PM
Last modification on : Thursday, January 7, 2021 - 3:46:09 PM
Long-term archiving on: : Thursday, October 11, 2018 - 1:57:27 PM


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  • HAL Id : tel-01834557, version 2



Nicolas Valette. Caractérisation fonctionnelle de petites protéines sécrétées chez les champignons lignolytiques. Biologie végétale. Université de Lorraine, 2017. Français. ⟨NNT : 2017LORR0324⟩. ⟨tel-01834557v2⟩



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