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Silica based materials for the encapsulation of β-Galactosidase

Abstract : The engineering of solid dietary supplements provides several advantages in the industrial formulation of food products, in terms of its production, storage and handling. Thereby, the goal of this doctoral work is to design bio-responsive carriers for the encapsulation of an exogenous enzyme able to catalyze the hydrolysis of lactose towards simple sugar molecules. In fact, there is a consensus that the onset of symptoms characteristic of lactose intolerance are associated with lactase deficiency in the small intestine. Providing the organism with exogenous lactase is the underlying application targeted by this work through the design of silicabased materials for encapsulation. The different types of bio-carriers developed had to overcome the simulated gastric conditions in order to release active enzyme molecules in the small intestine. Amorphous porous silica is a very good and non-toxic component affording protection versus acidic conditions, while providing controlled release. This inorganic material approved by the US Food and Drug Administration (FDA) has a relatively low cost, and presents a controlled structure (shape, size, pore diameter), as well as tunable surface chemistry. In agreement with the main objectives, four bio-adapted encapsulation strategies were investigated as potential routes to produce solid dietary supplements for lactose intolerance treatment: (i) physical entrapment of the enzyme in pre-synthesized meso-macroporous silica materials, (ii) physical entrapment of the enzyme in low porosity silica particles coated by liposomes, (iii) encapsulation of the enzyme into thermosensitive solid lipid nanoparticles (SLNs) (iv) encapsulation of the enzyme into a biopolymer matrix coated in a mesoporous silica shell
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Ileana-Alexandra Pavel-Licsandru. Silica based materials for the encapsulation of β-Galactosidase. Chemical engineering. Université de Lorraine, 2017. English. ⟨NNT : 2017LORR0322⟩. ⟨tel-01834554⟩

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