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Thermodynamique de l'assemblage de nano-structures et d'origami d'ADN

Abstract : DNA is the support of genetic information. The property of self-assembly of two complementary single strands to form a double helix enable the use of this biopolymer as a building block for nanofabrication. DNA origami are a method which enable the self-assembly of 2D or 3D nanostructures. In this method, a long single-stranded DNA taken from the genome of a phage is folded on itself in a programmable way thanks to a lot of short synthetic DNA strands. The design of origami is based on thermodynamic and on the optimization of the base pairing in the structures. However, although interactive tools that facilitate the design of DNA nano-structures have been developed, we know little about the folding process and its optimization. In this work, we study the thermodynamics of DNA nanostructures in order to have a better understanding of the folding process and to identify the key steps.We performed differential scanning calorimetry (DSC) on model structures and DNA origami. Thus, we have been able to identify the presence of key steps in the folding of small nanostructures. We show that by changing the sequences of the strands, it is possible to change the cooperativity and the stability of the assembly of the nanostructure and thus change the folding path.The study of small origami with one or two staples allowed us the see the influence of the position of the staples, of the sizes of the loops and of the orientation of the staples on the thermodynamic of the folding.Finally, the calorimetric measurements performed on origami allowed us to solve the collective hybridization of staple sets. This enable us to prioritize the origami assembly into separate domains.This work also consisted of the development of innovative methods of ultra-sensitive nano-calorimetry integrating microfluidics. These calorimetric methods will give us the access to the kinetic parameters of the folding and to the equilibrium thermodynamic parameters.Our results obtained on model nano-structures show that it is possible to optimize the design of DNA nanostructures by integrating the assembly process in the design of the structures. Such high-performance DNA nanostructures may allow in the future the development of molecular robot which is a very promising application of DNA nanostructures.
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https://tel.archives-ouvertes.fr/tel-01831617
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Submitted on : Friday, July 6, 2018 - 9:39:07 AM
Last modification on : Wednesday, October 14, 2020 - 4:16:27 AM
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  • HAL Id : tel-01831617, version 1

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STAR | CNRS | UGA | NEEL

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Clothilde Coilhac. Thermodynamique de l'assemblage de nano-structures et d'origami d'ADN. Science des matériaux [cond-mat.mtrl-sci]. Université Grenoble Alpes, 2018. Français. ⟨NNT : 2018GREAY007⟩. ⟨tel-01831617⟩

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