Skip to Main content Skip to Navigation
Theses

Application de procédés éco-responsables pour l’extraction de molécules de la macroalgue Solieria chordalis, caractérisations chimiques et étude d'activités biologiques

Abstract : This thesis has been carried out within the Laboratoire de biotechnologie et Chimie Marines. Theproject is an outcome of the collaboration of two regional companies, Armen Instruments and Newonat with an international research institute, CINVESTAV, Mexico. The red algae, Solieria chordalis (Rhodophyta, Gigartinales) is a proliferative seaweed native in the southern coast of Brittany. Every year, on the Rhuys peninsula in Morbihan, S. chordalis grows abundantly which represents a large amount of biomass, being still unused. The aim of this project, focused on the application of different ecofriendly processes (Supercritical carbon dioxide, Enzymatic Assisted Extraction and Microwave Assisted Extraction) used for the extraction of biocompounds from S. chordalis. Under the first part, the Microwave Assisted Extraction allowed to produce low molecular weight polysaccharide extracts with no cytotoxicity. These extracts showed an improved antiviral efficiency compared to those obtained by the conventional method. The second part focused on the interaction between different processes: Supercritical CO2, Enzymatic Assisted Extraction (EAE) and Microwave Assisted Extraction (MAE). The sequences of the processes allowed to propose a biorefinery perspective. The last part dealt with the optimization of fractionating method by Centrifugal Partition Chromatography (CPC). This innovative purification system led to isolate natural compounds such as Mycosporine-like Amino Acids with different properties, which can be valorized in the cosmetic field. This work enriches the knowledge of the seaweed S. chordalis and open a way of upgrading the proliferative biomass on the Brittany coasts.
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-01823710
Contributor : Abes Star :  Contact
Submitted on : Tuesday, June 26, 2018 - 1:19:06 PM
Last modification on : Friday, September 25, 2020 - 3:36:04 AM
Long-term archiving on: : Wednesday, September 26, 2018 - 9:43:11 PM

File

2017theseBoulhoR.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01823710, version 1

Collections

Citation

Romain Boulho. Application de procédés éco-responsables pour l’extraction de molécules de la macroalgue Solieria chordalis, caractérisations chimiques et étude d'activités biologiques. Biotechnologie. Université de Bretagne Sud, 2017. Français. ⟨NNT : 2017LORIS465⟩. ⟨tel-01823710⟩

Share

Metrics

Record views

751

Files downloads

434