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Influence de l'architecture macroporeuse en phosphate de calcium sur le comportement cellulaire in vitro

Abstract : Calcium phosphates such as β-TCP have been used for decades as synthetic bone substitutes. Its good chemical properties and its similar behavior to that of the bone in vivo and in vitro can be enhanced by the chosen shaping method. It is nowadays largely accepted that an optimized porous architecture will have a positive impact on the material's bioactivity. This study aims at studying the links between a porous architecture and cell proliferation and differentiation. β-TCP was manufactured by aqueous precipitation. Synthesis parameters were optimized in order to get a product complying with ISO 13779 and 13175 requirements. Three shaping methods were chosen for their ability to generate original structures. The impregnation of a polymeric scaffold yields a network of interconnected spherical pores (PS), stereolithography yields a network of interconnected cubical pores (3D) and ice templating yields a network of parallel ellipsoidal channel-like structure (CO). Two different trends emerged from the human mesenchymal stem cell culture: PS and 3D favored cell proliferation whereas CO promoted cell penetration and alkaline phosphatase activity. The latter is stimulated by β-TCP and this ability is enhanced by freeze casting. This could be explained by the state of cell differentiation: cells on CO samples seem to be far more differentiated than the other ones. However the study of key genes expression is needed to confirm this hypothesis.
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Submitted on : Wednesday, July 4, 2018 - 9:47:11 AM
Last modification on : Thursday, February 13, 2020 - 10:18:02 AM
Document(s) archivé(s) le : Monday, October 1, 2018 - 10:30:37 AM


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  • HAL Id : tel-01822768, version 2



Shaan Chamary. Influence de l'architecture macroporeuse en phosphate de calcium sur le comportement cellulaire in vitro. Autre. Université de Valenciennes et du Hainaut-Cambresis, 2018. Français. ⟨NNT : 2018VALE0006⟩. ⟨tel-01822768v2⟩



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