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Etude des phénomènes d'adhésion entre des cellules B et des gouttes d'huile fonctionnalisées par des anticorps à l'aide de pièges microfluidiques

Abstract : In the immune system, B cell activation is triggered by the specific binding of the B cell receptors with the antigens present on antigen-presenting cells like macrophage, dendritic cells, ... This recognition leads to an accumulation of adhesive proteins at the contact. This molecular structure is called immune synapse, from which the antigens are extracted by the B cell. The precise biological mechanisms implied in the extraction of antigens are still unknown. In our work, we propose to use antibody-coated oil droplets as substrates for the activation of B cells. These droplets have the advantage to have a liquid surface, allowing the antibodies-coated diffusion at their surface. Moreover, these droplets are potentially deformable, so they behave as cellular force probes. Lastly, the droplets have the same size of the B cells, so they mimic the antigen-presenting cells. In order to put in contact the B cells with the droplets, we fabricated a regular network of microfluic traps with U-shape in a microfluic chamber. The shape of the chamber has been optimized to guaranty a good trapping efficiency. Using the traps, we have been able to put into contact one antibody-coated droplet with one B cell in several traps in parallel, and to observe the contact in function of time by fluorescence microscopy. We observed an accumulation of the antibodies coated on the droplet at the surface of contact. The kinetics of accumulation has been measured and the time scale of accumulation has been deduced. Also, observations with fluorescence showed a polarization of the lysosomes near the contact, suggesting a B cell response.
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https://tel.archives-ouvertes.fr/tel-01821875
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Submitted on : Saturday, June 23, 2018 - 1:02:05 AM
Last modification on : Sunday, October 25, 2020 - 1:51:34 PM
Long-term archiving on: : Wednesday, September 26, 2018 - 6:58:06 PM

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  • HAL Id : tel-01821875, version 1

Citation

Olivier Mesdjian. Etude des phénomènes d'adhésion entre des cellules B et des gouttes d'huile fonctionnalisées par des anticorps à l'aide de pièges microfluidiques. Chimie-Physique [physics.chem-ph]. Université Pierre et Marie Curie - Paris VI, 2017. Français. ⟨NNT : 2017PA066502⟩. ⟨tel-01821875⟩

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