, Supporting information S1 Fig. DnaJ-1 and MLF interact in Kc167 cells

, A) Confocal images of fluorescent immunostainings against GFP (green) and HA (red) in

, Kc167 cells transfected with expression plasmids for GFP-DnaJ-1 and HA-MLF. Nuclei were stained with Topro3. Merged and individual channels are displayed

, Western blots showing the results of an immunoprecipitation experiment against GFP in Kc167 cells transfected with expression plasmids for the indicated proteins

, S2 Fig. LZ interacts with MLF and DnaJ-1

, A) Autoradiogram showing the results of pull down assays between in vitro translated 35 Smethionine-labeled LZ and the indicated GST fusion proteins

C. (. , Western blots showing the results of immunoprecipitation experiments against GFP performed in Kc167 cells transfected with expression vectors for GFP-LZ and various HA-MLF (B) or HA-DnaJ-1 (C) mutants

, S9 Fig. LZ expression increases with lz>GFP + cell size

, Quantification of LZ and NICD expression levels in lz>GFP + circulating blood cells of lz-GAL4, UAS-mCD8GFP/+ third instar larvae. Cells were pooled into 5 categories according to their size (% of the mean cell size) and LZ or NICD expression level in each pool was plotted

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