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Structuration morphologique et microbiologique des biofilms multi-espèces : de l’adhésion au biofilm mature

Abstract : Biofilms are a biological mode of life widely spread in both natural and engineered environments. In the last case, whether the biofilm is beneficial or detrimental for the process under consideration, both morphology and microbial community of the biofilm determine its impact. The objective of this thesis is to deepen our knowledge of biofilm structuring and, as a further goal, optimize a given process. Turbulent flows and multi-species consortia were used in order to better mimic industrial conditions. The first part of the project focused on the impact of shear stress on microbial adhesion. Results have demonstrated a gradual shift in bacterial communities with shear and a change in the spatial distribution of adhered microorganisms. Secondly, the work dealt with biofilm development. A memory effect, defined as the conservation of initial morphological and microbiological features despite a change in the environmental conditions, has been observed. Finally, a method for quantification of moving predators in mature biofilms has been developed. These predators actively shape the biofilm and their quantification is valuable, especially for wastewater treatment.
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  • HAL Id : tel-01810953, version 1

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Thibaut Saur. Structuration morphologique et microbiologique des biofilms multi-espèces : de l’adhésion au biofilm mature. Génie des procédés. Université Montpellier II - Sciences et Techniques du Languedoc, 2014. Français. ⟨NNT : 2014MON20174⟩. ⟨tel-01810953⟩

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