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Génération de cellules souches pluripotentes induites de patients Alzheimer et production d'un modèle de culture en trois dimensions de neurones pour les recherches diagnostiques et thérapeutiques de la maladie d’Alzheimer

Abstract : Amyloid precursor protein (APP) and Tau protein are two main molecular actors of the Alzheimer’s disease (AD), which is of prime importance in Human Health. Intensive research is ongoing to understand these proteins’ metabolism, action and implication in the pathological mechanism of these affections. They are the target of most therapeutic approaches and are used for biological diagnosis. In the present PhD project, our objective was to investigate neuronal APP and Tau protein processing and metabolism using biochemical tools and innovative multiplex immunodetection system (MSD®) in diverse cell culture models of AD. The goal was to get a comprehensive view oh physiopathological processes based on the analysis of samples generated in neuronal differentiated human embryonic stem cell and induced pluripotent stem cells derived from AD-patients. We generated several cell lines from an healthy control individual, and AD patients showing sporadic and familial forms of the disease. This project offer the unique opportunity to combine state-to-the-art approaches to understand how the APP fragments and peptides are generated as well as the modifications of the Tau protein in normal and pathological situation.
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https://tel.archives-ouvertes.fr/tel-01809514
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Submitted on : Wednesday, June 6, 2018 - 5:15:30 PM
Last modification on : Tuesday, September 8, 2020 - 4:08:28 AM
Long-term archiving on: : Friday, September 7, 2018 - 2:58:37 PM

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  • HAL Id : tel-01809514, version 1

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Laura Auboyer. Génération de cellules souches pluripotentes induites de patients Alzheimer et production d'un modèle de culture en trois dimensions de neurones pour les recherches diagnostiques et thérapeutiques de la maladie d’Alzheimer. Santé. Université Montpellier, 2018. Français. ⟨NNT : 2018MONTT004⟩. ⟨tel-01809514⟩

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