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La chromatographie en phase supercritique couplée avec une détection UV et spectrométrie de masse haute résolution pour l’analyse d’échantillons complexes : application aux bio-huiles

Abstract : This thesis focused on the analysis of bio-oils by supercritical fluid chromatography (SFC) in coupling with a UV detector and a high resolution mass spectrometer (HRMS). Still unknown, this technique is currently not widespread in comparison with gas chromatography. First, an optimization based on the maximization of peak capacity during the analysis of the bio-oil has been put in place. Pressure, temperature, stationary phases and mobile phases were selected to separate a maximum of peaks (more than a hundred peaks) on the widest possible retention window. Once separation optimized, an experimental work of coupling with the mass spectrometry was carried out first on a single quadrupole detector then on a time detector of flight with a linear trap (IT-ToF/MS). MS/MS fragmentation experiments were then carried out to propose an identification of the detected species. The reprocessing of the large number of data generated also necessitated the development of a reprocessing tool adapted to carry out the identification of molecular formulae and to propose developed structures using fragmentation and open-source comparison tools. The last part of this work was devoted to the comparison of the results obtained on a bio-oil by SFC-UV/HMRS, GCxGC-FI/MS and FT-ICR/MS. All of this methodological work has improved our knowledge of this type of SFC-UV / HRM coupling through application examples related to the characterization of ex-biomass complex matrices
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Submitted on : Tuesday, June 5, 2018 - 10:30:07 AM
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Julien Crepier. La chromatographie en phase supercritique couplée avec une détection UV et spectrométrie de masse haute résolution pour l’analyse d’échantillons complexes : application aux bio-huiles. Chimie analytique. Université de Lyon, 2018. Français. ⟨NNT : 2018LYSE1050⟩. ⟨tel-01807763⟩

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