Skip to Main content Skip to Navigation
Theses

Interaction des triterpènes avec les membranes synthétiques et l’albumine humaine : application aux progestatifs et corticostéroïdes et à deux structures pentacycliques

Abstract : The triterpenoids are a large and structurally diverse group of natural products derived from squalene. Progesterone derivatives and glucocorticoids are a group of oxygenated triterpenes having a tetracyclic skeleton and identified for their therapeutic properties. Whereas, erythrodiol and uvaol are pentacyclic triterpenes, known for their beneficial effects on human diet. In this thesis, we studied their interaction with the membranes of lipid vesicles and with human serum albumin to better understand their pharmacological properties. DSC, Raman spectroscopy, FTIR and fluorescence polarization of DPH were used to investigate the effect of triterpenes on the membrane fluidity. Besides, we used fluorescence spectroscopy to study the binding of cholesterol, a series of progesterone derivatives and another series of glucocorticoids to albumin. The results revealed that progesterone derivatives, glucocorticoids, erythrodiol and uvaol changed the physical properties of the bilayers. Progesterone derivatives and glucocorticoids have been proven to bind moderately to albumin. Dydrogesterone showed the highest binding constant. Finally, our study demonstrated that cholesterol exhibited a much weaker interaction with albumin compared to progesterone derivatives and glucocorticoids.Our work has led to a better understanding of triterpenes molecular mechanisms of their interaction with proteins and biological membranes and structural features controlling these interactions
Document type :
Theses
Complete list of metadatas

Cited literature [183 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01806331
Contributor : Abes Star :  Contact
Submitted on : Saturday, June 2, 2018 - 1:06:22 AM
Last modification on : Wednesday, November 20, 2019 - 3:27:23 AM
Long-term archiving on: : Monday, September 3, 2018 - 3:40:23 PM

File

TH2016AbboudRolaVExpurgee.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01806331, version 1

Collections

Citation

Rola Abboud. Interaction des triterpènes avec les membranes synthétiques et l’albumine humaine : application aux progestatifs et corticostéroïdes et à deux structures pentacycliques. Biochimie, Biologie Moléculaire. Université de Lyon, 2016. Français. ⟨NNT : 2016LYSE1305⟩. ⟨tel-01806331⟩

Share

Metrics

Record views

211

Files downloads

285