Skip to Main content Skip to Navigation
Theses

Imagerie moléculaire 3D quantitative des tissus en utilisant la microscopie Raman cohérente sans marquage

Rafael Canonge 1, 2
2 MOSAIC - MOSAIC
FRESNEL - Institut FRESNEL
Abstract : This thesis focuses on multiphotonic microscopy techniques development and use in order to image human biological samples. A multiphotonic imaging setup using label-free nonlinear contrasts mechanisms such as two-photons fluorescence, second harmonic generation, or stimulated Raman effect (CARS or SRS) has been designed and developped during this PhD, and I present the experimental work in two main research topics.In a first part, we compare label-free 3D imaging with classic histological imaging using colorimetric labels in human digestive system. We show that multiphotonic technics allow to reconstruct the organization and discern the molecular compounds inside the tissues, in order to get a caratérization of the cancerous tumors developpement.The second part is related to the application of our multimodal setup to the quantitative study of real active molecular compounds real time penetration into in vivo human skin. We show that multiphotonic microscopy make possible to mesure active molecules in depth 3D concentration in the skin in order to understand transcutaneous diffusion mechanisms in cosmetic and pharmacological applications.
Document type :
Theses
Complete list of metadatas

Cited literature [66 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01798088
Contributor : Abes Star :  Contact
Submitted on : Wednesday, May 23, 2018 - 10:29:07 AM
Last modification on : Wednesday, February 12, 2020 - 7:29:49 PM
Long-term archiving on: : Friday, August 24, 2018 - 7:25:39 PM

File

these.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01798088, version 1

Collections

Citation

Rafael Canonge. Imagerie moléculaire 3D quantitative des tissus en utilisant la microscopie Raman cohérente sans marquage. Optique [physics.optics]. Ecole Centrale Marseille, 2017. Français. ⟨NNT : 2017ECDM0010⟩. ⟨tel-01798088⟩

Share

Metrics

Record views

210

Files downloads

506