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Systèmes biomimétiques pour l'étude du changement de forme cellulaire

Abstract : Intracellular transport involves membrane compartments and thus requires dynamic changes in the morphology of cell membranes. In this case, membrane tubes are formed whose radius is of the order of several tens of nanometers. We develop biomimetic systems based on model lipid membranes to decipher the mechanisms of membrane remodelling in particular under the action of the actin cytoskeleton. The mechanics of membrane nanotubes, especially the force needed to form and maintain a nanotube, are now well understood. The force depends on the curvature elasticity of the membrane and on its mechanical tension that is controlled in our experiment by micropipette aspiration. By using a four-quadrant diode, we obtain an unprecedented temporal resolution, in the order of 4 µs, and a force resolution under pN. This setup allows us to access unrivaled membrane nanotube properties.This thesis paves the way for studying the effect of actin dynamics on membrane nanotubes
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Submitted on : Wednesday, May 9, 2018 - 12:10:11 PM
Last modification on : Tuesday, July 21, 2020 - 3:58:22 AM
Long-term archiving on: : Tuesday, September 25, 2018 - 2:22:08 PM


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  • HAL Id : tel-01788669, version 1


Fabrice Valentino. Systèmes biomimétiques pour l'étude du changement de forme cellulaire. Biophysique. Université Sorbonne Paris Cité, 2016. Français. ⟨NNT : 2016USPCC208⟩. ⟨tel-01788669⟩



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