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Développement de sondes chimiluminescentes pour la détection d'activités enzymatiques

Abstract : Since several years, it is easier to exploit biological phenomena in cellulo through technologies dealing with bioimaging. This method gathers fluorescence, bioluminescence and chemiluminescence. Even though fluorescence is the most employed technic, bioluminescence and chemiluminescence, being the consequence of (bio)chemical reaction, have been widely studied for decades. In fact, they can avoid the main problems encountered in fluorophore use: exciting light. Chemiluminescence is thus the appropriate approach to avoid biological autofluorescence. 1,2-dioxetane is one of the moieties that, upon decomposition, can generate an excited state on a connected fluorophore, giving rise to its intrinsic luminescence. The aim of our project was to develop and study new 1,2-dioxetan chemiluminescent probes based on phenol or naphthol moieties, for targeted enzymatic complexes detection with in cellulo and in vivo bioimaging. The strategy relied on the synthesis of chemiluminescent scaffolds comprising thermally stable 1,2-dioxetan, on which the trigger and the connected NIR fluorophore can be easily diversified. Two methods have been attempted: 1) coupling to a water-soluble version of a known fluorophore (i.e. Nile red) allowing an energy transfer through bonds (i.e. TBET) 2) connection with a lanthanide complex giving rise to an energy transfer through space (i.e. CRET).
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Submitted on : Tuesday, April 3, 2018 - 4:12:07 PM
Last modification on : Tuesday, September 1, 2020 - 3:03:44 AM


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  • HAL Id : tel-01757325, version 1


Kathleen Solmont. Développement de sondes chimiluminescentes pour la détection d'activités enzymatiques. Chimie organique. Normandie Université, 2018. Français. ⟨NNT : 2018NORMR003⟩. ⟨tel-01757325⟩



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