Biosensor activatable in both fluorescence and 129Xe NMR for detection of recombinant proteins

Abstract : Full understanding of intracellular phenomena involves sensitive and non-invasive detection. A less disruptive method than labeling with fluorescent proteins uses binding between a tag of only six natural amino acids that can be genetically incorporated into the protein of interest and a small molecule called FlAsH. This molecule has the ability to fluoresce only when it binds to its tetracysteine target. Another technique based on 129Xe NMR has emerged. Xenon is hyperpolarized to enhance the NMR signal by orders of magnitude and its reversibleencapsulation in functionalized host systems gives it a specific spectral signature. Capability of the noble gas to cross cell membranes without losing its polarization enables in cellulo investigations.This doubly smart probe is highly promising for monitoring, studying, detecting recombinant proteins. Structural, chemical and lateral resolutions are combined by the bimodality of this new concept, which can be extended to in cellulo detection.
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Emilie Mari. Biosensor activatable in both fluorescence and 129Xe NMR for detection of recombinant proteins. Analytical chemistry. Université Paris-Saclay, 2017. English. ⟨NNT : 2017SACLV067⟩. ⟨tel-01733118⟩

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