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In vivo and in vitro study of pancreatic islets aging : impact of endothelial senescence and microparticles on islet function

Abstract : This scientific work has tackled the question of the pancreatic islets aging and the effect of endothelial senescence and microparticles (MPs) on islet function. We investigated the impact of aging on pancreas morphology, fate and on the function of the pancreatic islet by comparative analysis between pancreas in young and middle-aged rats, as well as the role of pro-senescent endothelial MPs on islet function and their premature senescence. Our in vivo data show that the pancreas is an early sensitive organ to oxidative stress accumulating with age and leading to overexpression of the procoagulant and senescence markers without appearance of apoptosis. In vitro, MPs of senescent endothelial cells have a pro-senescent effect on pancreatic islets isolated from young rats with characteristic SA-β-galactosidase activity, overexpression of p53, p21 and p16 markers and reducing the ability of insulin secretion in response to glucose. Altogether, our in vivo and in vitro data indicate the contribution of endothelial senescence as a possible contributor to graft dysfunction.
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https://tel.archives-ouvertes.fr/tel-01726953
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Submitted on : Thursday, March 8, 2018 - 4:43:03 PM
Last modification on : Tuesday, October 20, 2020 - 11:31:32 AM
Long-term archiving on: : Saturday, June 9, 2018 - 3:37:46 PM

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  • HAL Id : tel-01726953, version 1

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Mohamad Kassem. In vivo and in vitro study of pancreatic islets aging : impact of endothelial senescence and microparticles on islet function. Endocrinology and metabolism. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAJ007⟩. ⟨tel-01726953⟩

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