Skip to Main content Skip to Navigation
Theses

Investigation of urine metabolic profiles in newborns with prenatally diagnosedunilateral urinary tract dilatation using 1H NMR spectroscopy and metabolomic analysis

Abstract : The prenatal finding of unilateral Urinary Tract Dilatation (UTD) can be transient or represent a significant urinary flow impairment that would lead to progressive deterioration of renal function. Identifying urinary biomarkers could help to differentiate uropathy requiring surgical management from transient dilatation at an early stage.Metabolic phenotyping studies provide untargeted quantification of all detectable low molecular-weight molecules by profiling without any a priori the metabolic signatures of biological samples in connection to physiopathological events.The main objective of this study is to identify diagnosis and prognosis urinary markers for uropathy in newborns with prenatally diagnosed unilateral UTD using 1H-NMR spectroscopy combined with multivariate statistical analyses.A total of 70 newborns with unilateral UTD and 90 controls were included in this prospective study. First, the usefulness of different ultrasound grading systems in predicting the need for surgical intervention is evaluated. Then, we report the absence of significant difference between the urinary metabolic profiles of newborns with UTD and controls. In thethird part, the influence of age, weight and height on the urinary metabolic profiles of healthy newborns is highlighted for the first time, and key-metabolites responsible for this evolution are identified. Finally, we demonstrate the influence of age on the urinary metabolic profiles of children with UTD. This work allows a deeper understanding of the metabolic maturation of healthy newborns. It contributes to identify potential confounding factors for metabolomics investigations in neonatology. It represents a step toward a better comprehension of thephysiopathology of UTD
Document type :
Theses
Complete list of metadata

Cited literature [60 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01724674
Contributor : Abes Star :  Contact
Submitted on : Tuesday, March 6, 2018 - 4:33:07 PM
Last modification on : Tuesday, June 15, 2021 - 2:30:15 PM
Long-term archiving on: : Thursday, June 7, 2018 - 2:23:11 PM

File

TH2017SCALABREAURELIEN.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01724674, version 1

Collections

Citation

Aurélien Scalabre. Investigation of urine metabolic profiles in newborns with prenatally diagnosedunilateral urinary tract dilatation using 1H NMR spectroscopy and metabolomic analysis. Analytical chemistry. Université de Lyon, 2017. English. ⟨NNT : 2017LYSE1272⟩. ⟨tel-01724674⟩

Share

Metrics

Record views

346

Files downloads

623