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Molecular approaches for the discovery, development and application of toxicity biomarkers in gammarids

Abstract : The routine use of biomarkers in environmental biomonitoring faces several drawbacks, especially in invertebrates. Among these limitations, the lack of validated species-specific biomarkers and multibiomarker methodologies comprise major constraints. Based on gene and protein catalogs obtained from proteogenomics experiments previously performed on the ecotoxicological-relevant species Gammarus fossarum, this thesis aimed at identifying and validating molecular biomarkers for the diagnostic of toxic perturbations in this species. Following recent methodologies for the development of biomarkers in human disease diagnosis, we implemented a fast, specific, quantitative multiplexed targeted proteomics assay (using Selected Reaction Monitoring mass spectrometry) to study dozens of protein biomarker candidates simultaneously. The assay was applied to assess the physiological importance of protein candidates, and to assess their pertinence as biomarkers after laboratory and field exposures to chemical contamination. The second part of this thesis aimed at the development of specific endocrine disruption biomarkers, through two complementary approaches. The first approach, based on a comparative shotgun proteomic analysis using a known endocrine disruptor for arthropods, allowed exhaustively increasing the whole-proteome analysis through the detection of roughly 4000 proteins (53 modulated by the exposure). The second comprised sequence homology searches, phylogenetic analyses, and gene expression studies for proposing new biomarker candidates identified from literature searches
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Submitted on : Monday, March 5, 2018 - 6:57:07 PM
Last modification on : Tuesday, October 20, 2020 - 11:30:25 AM
Long-term archiving on: : Wednesday, June 6, 2018 - 4:39:37 PM


Version validated by the jury (STAR)


  • HAL Id : tel-01723905, version 1
  • IRSTEA : PUB00056771



Duarte Domingos Gouveia. Molecular approaches for the discovery, development and application of toxicity biomarkers in gammarids. Ecotoxicology. Université de Lyon, 2017. English. ⟨NNT : 2017LYSE1318⟩. ⟨tel-01723905⟩



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