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Primitive macrophages regulate the development of dorsal root ganglia sensory neurons in mouse embryos

Abstract : The primitive macrophages invade the spinal cord as early as E11.5-12.5 in the mouse embryo. These cells interact with growing spinal neurites of the dorsal root ganglia sensory neurons as early as E12.5. Microglia is known to regulate several developmental processes in the central nervous system, but their role in the development of the peripheral nervous system remains unknown. We show that primitive macrophages regulate the development of sensory neurons in the mouse embryo. We first discovered that primitive macrophages interact with peripheral neurites of sensory neurons as early as E11.5. We then demonstrated that the absence of macrophage in PU.1KO mice or their immunopharmacological ablation resulted in an initial reduction of TrkB+ and TrkC+ neurons at E11.5, followed by a transient increase in their number at E12.5. This increase is associated with a transient increase in their developmental death which would explain why their numbers become normal again from E13.5. In macrophage-free embryos, death increases again to E15.5 for TrkC+ neurons and as early as E14.5 for TrkB+ neurons. Concerning TrkA+ neurons, their number remains deficient between E12.5 and E15.5, although their developmental death is not affected. We have finally shown that cell proliferation was decreased at E12.5 in ganglions of PU.1KO mice. These results are the first demonstration that primitive macrophages and / or immature microglia can also regulate the embryonic development of the peripheral nervous system.
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https://tel.archives-ouvertes.fr/tel-01722781
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Submitted on : Monday, March 5, 2018 - 9:57:10 AM
Last modification on : Monday, August 31, 2020 - 12:26:21 PM
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  • HAL Id : tel-01722781, version 1

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Monara Kaélle Angelim. Primitive macrophages regulate the development of dorsal root ganglia sensory neurons in mouse embryos. Neurons and Cognition [q-bio.NC]. Université Pierre et Marie Curie - Paris VI, 2017. English. ⟨NNT : 2017PA066304⟩. ⟨tel-01722781⟩

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