Skip to Main content Skip to Navigation

Assemblage permanent de micro-objets par diélectrophorèse associée à une méthode de couplage covalent

Abstract : The design of micro and nanomaterials with controlled properties requires the development of new bottom- up assembly approaches. The work presented in this manuscript aims to control the formation of aggregates of colloidal particles and cells, with potential applications in the fields of biosensors, microelectronics, optics and tissue engineering. The proposed approach for assembling colloids into organized structures is based on the use of dielectrophoresis, a phenomenon observed when polarizable particles are placed in a non-uniform electric field. One of the drawbacks of this technique is the reversibility of the assemblies thus formed, the cancellation of the electric field causing the redispersion of the colloids. As a solution to this problem, we proposed to use a coupling agent to maintain the cohesion of the structures formed in the liquid medium. In order to better control the dielectrophoretic behavior and the chemical reactions exploited, electrical and chemical characterizations of the manipulated particles were carried out. Moreover, different types of microelectrodes have been studied for the generation of the electric field required for the assembly. A new approach has been developed for the fabrication of transparent micro patterned ITO microelectrodes and their integration in microfluidic systems, based on the exploitation of a thin micro-structured PDMS membrane used as an insulating layer. The method has been applied to the fabrication of "vertical" microelectrodes and of quadrupolar electrode arrays. The latter were used to obtain permanent assemblies of carboxylic acid functionalized, fluorescent, polystyrene particles, by combining negative dielectrophoresis with the use of a chemical coupling agent (Jeffamine). HEK 293 cell aggregates were also produced by negative dielectrophoresis. We have demonstrated that it was possible, under certain conditions, to preserve the permanent character of the cell aggregates after field removal.
Document type :
Complete list of metadata

Cited literature [132 references]  Display  Hide  Download
Contributor : Abes Star :  Contact
Submitted on : Thursday, February 22, 2018 - 3:02:05 PM
Last modification on : Monday, September 13, 2021 - 2:44:04 PM
Long-term archiving on: : Wednesday, May 23, 2018 - 1:35:09 PM


Version validated by the jury (STAR)


  • HAL Id : tel-01715239, version 1


Samia Menad. Assemblage permanent de micro-objets par diélectrophorèse associée à une méthode de couplage covalent. Autre. Ecole Centrale de Lyon, 2014. Français. ⟨NNT : 2014ECDL0037⟩. ⟨tel-01715239⟩



Record views


Files downloads