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Intégration de matériaux nanostructurés dans la conception et la réalisation de biocapteurs sans marquage pour la détection de cibles d'intérêt

Abstract : The main purpose of this work was the design and the development of biosensors by using non-marking transduction methods, such as electrochemical impedance spectroscopy (EIS), for the detection of targets of interests. To this end, various molecular architectures have been developed to allow the transduction of the signal resulting from the recognition between the bioreceptor and its substrate, and thus lead to the detection of the target.The systems developed are based on the integration of nanomaterials, such as carbon nanotubes or tungsten disulfide, to ensure the immobilization of the biospecific entity at the surface of the sensor. The advantages of these materials are multiples, since they allow a very large increase in the specific surface area and are also used in the functionalization of the surface of the electrode. Indeed, one of the major challenges encountered in the development of biosensors is the strategy involved in the immobilization of the biospecific entity on the surface of the sensor.This work was initially interested in the realization and characterization of thin films of these nanomaterials as well as their transfer to the surface of an electrode. In this context, the aim is to design porous bioarchitectures based on electrogenerated functional polymers around carbon nanostructures allowing the penetration of large biomolecules such as antibodies to develop high-performance immunosensors.The second part of the work was oriented towards the design of biosensors using these different materials. The reliability of the process has been validated by the design of immunological systems for the detection of the anti-cholera toxin antibody and dengue toxin antibody.Finally, a last enzymatic biosensor, based on the use of tungsten disulfide nano-sticks, has been developed. The latter allows the detection of two molecules of interest, catechol and dopamin, by the use of polyphenol oxidase.
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https://tel.archives-ouvertes.fr/tel-01715215
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Submitted on : Thursday, February 22, 2018 - 2:48:05 PM
Last modification on : Wednesday, October 14, 2020 - 3:54:14 AM
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Quentin Palomar. Intégration de matériaux nanostructurés dans la conception et la réalisation de biocapteurs sans marquage pour la détection de cibles d'intérêt. Chimie théorique et/ou physique. Université Grenoble Alpes, 2017. Français. ⟨NNT : 2017GREAV069⟩. ⟨tel-01715215⟩

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