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Integration of functions dedicated to the mechanical solicitation and characterization of biological cells in microfluidic devices

Amin Amirouche 1
1 INL - LOCI - INL - Lab-On-Chip et Instrumentation
INL - Institut des Nanotechnologies de Lyon
Abstract : This thesis aims at evaluating two different techniques for the distinction of mechanically impaired biological samples. Both approaches were developed and investigated keeping in mind the long term objective which is the application of these approaches to diagnosis based on cell mechanical phenotype. Although, we developed these approaches on Red Blood Cells as as their mechanical properties can be affected by important pathologies, all the concepts developed in this work can be adapted to other cell types.We characterized mechanically RBCs using a passive microfluidic technique. We focused on the shape relaxation of healthy Red Blood Cells (hRBCs) flowing out of an oscillating width channel and demonstrated the dependency of their mechanical response upon the experimental conditions. We reported the existence of two relaxation modes for RBCs depending on the flow parameters, we used the interface between the two modes to extract the mechanical properties of RBC.Using a second approach, we presented the results obtained during the study of the mechanical behavior of hRBCs using electrodeformation assays. We investigated the influence of the solicitation parameters on the cell response and concluded on optimized conditions for cell solicitation without altering them. Finally, we evaluated both techniques in the discrimination of rigidified samples from healthy couterparts. Advantages and drawbacks of both techniques were discussed
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Amin Amirouche. Integration of functions dedicated to the mechanical solicitation and characterization of biological cells in microfluidic devices. Biomechanics [physics.med-ph]. Université de Lyon, 2017. English. ⟨NNT : 2017LYSE1226⟩. ⟨tel-01707400⟩

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