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Biotechnological approaches of the expression and diversity of the plant mitochondrial genome

Abstract : The mitochondrial DNA of plants is dynamic and its expression is complex. Using a strategy based on the natural import of nuclear-encoded transfer RNAs from the cytosol, we targeted to mitochondria in Arabidopsis thaliana the orf77 RNA characteristic for S-CMS in maize and we analyzed the effects on the transcriptome. The results showed that the mitochondrial transcriptome is tighly regulated during plant development and is strongly buffered at early stages. Mitochondrial targeting of orf77 also triggered a cross-talk with the nucleus. On the other hand, DNA replication and repair in plant mitochondria involve active recombination controled by nuclear-encoded factors. We identified a new member of this set of factors, the 5'-3' exonuclease potentially responsible for the resection of DNA ends in recombination-mediated repair of double-strand breaks. As a whole, the results open prospects for generating mitochondrial genetic diversity and creating CMS lines with agronomical interest.
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Submitted on : Monday, February 12, 2018 - 5:07:07 PM
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  • HAL Id : tel-01707357, version 1

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Rana Khalid Iqbal. Biotechnological approaches of the expression and diversity of the plant mitochondrial genome. Genomics [q-bio.GN]. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAJ035⟩. ⟨tel-01707357⟩

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