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Mesure non invasive de suivi des transferts de fluides liés aux activités cardiorespiratoires chez le rat : vers une «bague aortique virtuelle»

Abstract : It is recognized that inductive plethysmographic signals contain cardiac and respiratory components, which can be of interest for physiological monitoring. This technology is widely used in humans and medium mammals, but it has never been implemented in small laboratory rodents. This PhD aims to provide the analytic and experimental proof (TRL3) of a cardiac application of the cardio-respiratory inductive plethysmography (CRIP), based on the upstream concept of an “virtual aortic probe” (VAP). The VAP allows to realize an extern measure of “instantaneous” aortic flows thanks to CRIP instead of an instrument located directly around the vessel.The PhD starts with a phase of specification and conception driven by the interdependency between physiology and instrumental constraints. The expected metrological performances are established by a scale jumping between man and rat. The development of an optimized acquisition line has enabled to stretch the limits of existing systems; it allows to measure volume variations of a few microliters. At the same time, validation criteria and reference methods have been identified and experimental protocols have been specified in order to define the validation strategy of the developed instrument and VAP concept.At the end of these 3 years, an ultra-high resolution CRIP system has been developed. After calibration, the accuracy on the section variation measurements is 5% on a micrometric test-bench. The interchangeability of the thorax volume variation measure on a physiological range has been evaluated by comparison with a pneumotachographic measure on 9 anesthetized animals and the limits of agreement are lower than 20%. A hemodynamic challenge has been induced on 11 anesthetized animals, and the aortic flow has been simultaneously measured by CRIP and with an ultrasonic flow probe at under diaphragm level. This demonstrates the equivalence between both systems. And the high similarity between flow signals from both methods validates the proposed concept of virtual aortic probe
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https://tel.archives-ouvertes.fr/tel-01692780
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Submitted on : Thursday, January 25, 2018 - 2:16:15 PM
Last modification on : Wednesday, October 7, 2020 - 1:20:04 PM
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Timothé Flenet. Mesure non invasive de suivi des transferts de fluides liés aux activités cardiorespiratoires chez le rat : vers une «bague aortique virtuelle». Zootechnie. Université Grenoble Alpes, 2017. Français. ⟨NNT : 2017GREAS013⟩. ⟨tel-01692780⟩

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