Thermographie multi-échelle par méthodes optiques sur gouttelettes et bulles micrométriques : applications aux nanoparticules irradiées par laser ultra-intense et aux édifices biomoléculaires en cours de déshydratation

Abstract : The aim of this thesis was to study the thermodynamics of small objects (nanoparticles or biomolecule) in out-of-equilibrium media. Two mirror media were explored, namely charged micrometric droplets and microbubbles generated under intense laser irradiation. By nature, these two systems are difficult to probe by traditional methods, which is why an innovative multi-scale thermography by optical methods has been developed. Thus, a local (within the nano-objects) and a global (in the surrounding medium) thermography were carried out. Such thermography uses Laser-Induced Fluorescence - a simple, non-invasive and efficient method for providing temperature measurements with good spatial, temporal and thermal resolution - using thermochromic dyes directly in solution or incorporated into nano- objects. This thermography is also supplemented with other physical measurements, in particular the size of microdroplets and microbubbles, towards a complete thermodynamic study of these systems. These thermodynamic studies were carried out around the two following themes: Biomolecular structures in charged microdroplets during dehydration. Electrospray sources, which have become an essential tool in mass spectrometry, present a rich and still poorly understood thermodynamics. Measurements of the size and temperature of charged micrometric droplets during evaporation have been possible in order to obtain a complete thermodynamic description of an electrospray source. Moreover, in order to be able to follow the conformation of biomolecules (proteins) during dehydration, the concept of fluorescence anisotropy as a conformational probe has been validated in solution and can be transposed for an in situ analysis into the electrospray plume. Nanoparticles in microbubbles generated by intense laser irradiation. Here, we want to study the influence of nanoparticles during the generation of microbubbles by nano-cavitation induced by intense laser irradiation. This thematic is at the heart of the project "ERTIGO". The main scientific objective of this project was to obtain an understanding of the absorption mechanisms of a solution containing irradiated nanoparticles. For this purpose, an atypical light source (random laser) has been used in order to be able to illustrate this complex out-of-equilibrium system as a function of time by optical microscopy. In parallel, a local measurement of the temperature of the nanoparticles is envisaged
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Antonin Soleilhac. Thermographie multi-échelle par méthodes optiques sur gouttelettes et bulles micrométriques : applications aux nanoparticules irradiées par laser ultra-intense et aux édifices biomoléculaires en cours de déshydratation. Optique [physics.optics]. Université de Lyon, 2017. Français. ⟨NNT : 2017LYSE1192⟩. ⟨tel-01688828⟩

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