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CARACTERISATION DE LA B-GLYCOSIDASE DE LA BLATTE PERIPLANETA AMERICANA : APPLICATION A LA VALORISATION DES GLYCOALCALOÏDES DE LA POMME DE TERRE EN DECOMPOSITION

Abstract : Potato produce glycoalkaloids as most plants of Solanaceae family. The principal glycoalkaloids, α-chaconine and α-solanine are produced in greater quantities when potato tubers are exposed to light and are subject to deteriorations. These compounds are toxic and can represent a real danger for the consumer and the environment where they are discharged during their degradation. In this work, the estimation of glycoalkaloids in the flesh of different types of decayed potatoes usually found in different market places of Abidjan (Ivory Coast) was evaluated. The results showed that turned green and also sprouting or rotting potato flesh contain high amounts of toxic solanine and chaconine, exceeding by 2 to 5-fold the recommended limit. For safety consideration, these decayed potatoes should be systematically set aside. The accumulation of α-chaconine and solanine in potatoes can be seen as an attractive source of solanidine that is an important precursor for hormone synthesis and some pharmacologically active compounds. To this end, we proposed herein a simple chemo-enzymatic protocol comprising a partial acidic hydrolysis followed by an enzymatic treatment with the β-glycosidase from Periplaneta americana whose gene was isolated from a cDNA genomic library in order to detoxify these compounds and produce solanidine.
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  • HAL Id : tel-01687832, version 1

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Grokore Yvonne Koffi. CARACTERISATION DE LA B-GLYCOSIDASE DE LA BLATTE PERIPLANETA AMERICANA : APPLICATION A LA VALORISATION DES GLYCOALCALOÏDES DE LA POMME DE TERRE EN DECOMPOSITION. Microbiologie et Parasitologie. INSA de Toulouse, 2016. Français. ⟨NNT : 2016ISAT0040⟩. ⟨tel-01687832⟩

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