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Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric fields : effective new tool to control mesenchymal stem cells spontaneous Ca2+ oscillations

Abstract : Pulsed electric fields are widely used in research, medicine, food industry and other biotechnological processes. The interaction of one 100 µs pulse with the plasma membrane and the endoplasmic reticulum membrane was evaluated in two different cell types. Pulse amplitude ranged between 100 and 3 000 V/cm. Organelles membrane permeabilization using this kind of pulses was experimentally demonstrated for the first time. The use of such a pulse to control the spontaneous calcium oscillations in human-adipose mesenchymal stem cells was also assessed. By creating electro-induced calcium spikes of different amplitudes, the pulse can either add a supplementary spike, or, on the contrary, inhibit the spontaneous oscillations for some tens of minutes. During this inhibition period, the electric pulse-mediated addition of calcium spikes of desired amplitude and frequency is still possible. The delivery of 100 µs pulses to stem cells undergoing osteodifferentiation was also performed. The electric pulse seemed to delay the differentiation. Moreover, during osteogenic differentiation, cells cultures displayed an organization in a few cell layers. The characterization of these layers gave results that may help to obtain mature osteoblast in less time than usual one. The use of the microsecond electric pulses technology to permeabilize the plasma and the internal cell membranes as well as to modulate internal calcium concentrations is therefore interesting to study the role of calcium in many physiological processes and to manipulate the cell calcium dynamics (oscillations, waves, spikes) in different cell types. Doing so, this available, simple and easy to apply technology could be used for the modulation and the control of basic cellular functions such as proliferation, differentiation and apoptosis.
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https://tel.archives-ouvertes.fr/tel-01687535
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Submitted on : Thursday, January 18, 2018 - 3:22:08 PM
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Hanna Hanna. Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric fields : effective new tool to control mesenchymal stem cells spontaneous Ca2+ oscillations. Cellular Biology. Université Paris Saclay (COmUE), 2016. English. ⟨NNT : 2016SACLS493⟩. ⟨tel-01687535⟩

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