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Mise au point et évaluation de nouveaux revêtements de stents pour application cardio-vasculaire

Abstract : The purpose of this work was to study the biocompatibility of a dextran-graft-polybutylmethacrylate copolymer coated on cobalt chromium metallic stent. This study was divided in 3 parts: 1/the production of the copolymer and its physico-chemical characterization; 2/ its in vitro evaluation and 3/ its in vivo evaluation in several models. In the first step, 2 copolymers with different concentrations were synthetized and shaped for the following experiments. Their FTIR examination, contact angle measurement and a first in vivo implantation to evaluate foreign body reaction lead to the selection of one copolymer: the Dex-PBMA. No chronicle inflammatory reaction was noticed. Dynamic tests and SEM observations of coated stents confirmed the presence and the resistance of the Dex-PBMA coating. In vitro tests showed both low bacterial and platelet adhesions and a moderate thrombogenicity. An ex vivo test under flow with a model molecule – the Tacrolimus – showed the ability of Dex-PBMA to deliver drug. In vitro, the human endothelial progenitors and mesenchymal stem cells adhesion and proliferation were low but didn’t reveal any toxic effect. Finally Dex-PBMA coated stent were implanted in vivo in a healthy rat aorta model of stenting then in a rabbit model of restenosis. In rat, the intimal hyperplasia was moderate and an endothelium was present 30 days after stent implantation. First rabbit implantation confirmed these trends nevertheless this study must be extended to obtain significant results. In conclusion, these data demonstrate that Dex-PBMA is an interesting material for stent coating.
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  • HAL Id : tel-01686255, version 1

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Cécilia Delattre. Mise au point et évaluation de nouveaux revêtements de stents pour application cardio-vasculaire. Autre. Université Sorbonne Paris Cité, 2015. Français. ⟨NNT : 2015USPCD061⟩. ⟨tel-01686255⟩

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