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Évaluation in vitro et in vivo des perturbateurs endocriniens chez le poisson zèbre : cas de substances seules et en mélanges

Abstract : This PhD thesis aims at assessing the potential of innovative in vitro and in vivo zebrafish based bioassays for biomonitoring of surface water contamination by xeno-estrogens. For this purpose, the bioassays were applied to assess the effect of environmentally relevant surface water pollutants, alone and in simple (artificial) and complex (environmental samples) mixtures. The screening of surface water samples in zebrafish- (ZELH-zfERβ2 cells) and human-based (MELNcells) bioassays revealed qualitative and quantitative differences which could not be entirely explained by the xeno-estrogens identified. To better understand the response of bioassays to complex environmental mixtures, the activity of environmentally relevant surface waterpollutants was characterized across the bioassays, alone and in 2 to 12-component mixtures combining estrogenic and non-estrogenic chemicals to simulate an environmental contamination.The results indicate that zebrafish-based bioassays have a different and even in some cases an opposite response to the simple mixtures compared with the human-based bioassay MELN. These differences are explained by different sensitivity to some pollutants leading to bioassay-specific interactions with estrogen receptor activation. Al together, this work shows that the biological particularities of each bioassay can influence the response to estrogenic chemicals when mixed with other environmental pollutants, opening the discussion regarding their implementation in chemical water biomonitoring.
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Submitted on : Monday, January 8, 2018 - 10:57:32 AM
Last modification on : Tuesday, October 20, 2020 - 11:31:31 AM
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  • HAL Id : tel-01677239, version 1



Hélène Serra. Évaluation in vitro et in vivo des perturbateurs endocriniens chez le poisson zèbre : cas de substances seules et en mélanges. Chimie analytique. Université de Bordeaux, 2017. Français. ⟨NNT : 2017BORD0744⟩. ⟨tel-01677239⟩



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