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Développement de stratégies de biofonctionnalisation de surface de nano-objets pour des applications biologiques

Abstract : The aim of this study was the design of nanoparticles for three different biologicalapplications: magnetic nanoparticles cluster for magnetic extraction of biological materials,multimodal contrast agents (MRI and near infrared fluorescence imaging) for atherosclerosisdiagnosis, and fluorescent silica nanoparticles with two different dyes for in vitro and in vivo tumorlabeling. One part of the project dealt with the developement of a new grafting method ofpoly(ethylene oxide) macromolecules onto nanoparticle’s silica surfaces (PEGylation) in order toobtain a high grafting densities. The obtained results have shown that this PEGylation reduces the nonspecificprotein adsorption allowing a better extraction and sorting efficiency, and also permitsnanoparticles to escape the surveillance of the immune system for in vivo tumor labeling. Therefore,the biomolecular recognition of the nanoparticles has been optimized by controlling the number ofconjugated biomolecules and by studying this biomolecular recognition using biophysical methods(SPR, QCM-D). Finally, the different nano-objects were evaluated in the context of their respectiveapplication.
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Laurent Adumeau. Développement de stratégies de biofonctionnalisation de surface de nano-objets pour des applications biologiques. Matériaux. Université de Bordeaux, 2015. Français. ⟨NNT : 2015BORD0425⟩. ⟨tel-01674168⟩

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