Skip to Main content Skip to Navigation
Theses

In vivo study of the suppression of cell-autonomous and systemic RNA silencing by the Peanut clump virus protein P15

Abstract : In plants, RNA interference (RNAi) is the main antiviral defense mechanism. It is initiated through the processing of viral RNA into 21-22nt long siRNA by DCL4 and DCL2, respectively. These siRNA can mediate sequence-specific local defense reactions (cell-autonomous RNAi) or move to distant tissues to prime defenses in naive cells (systemic RNAi). Consequently, viruses have evolved proteins (VSRs) to suppress both aspects of RNAi. In this in vivo study, I show that P15, the VSR of Peanut clump virus (PCV), binds and sequesters both 21nt and 22nt siRNA. Importantly, it stops the movement of 22nt siRNA more efficiently than 21nt siRNA. During infection, P15 is shuttled into peroxisomes, and is able to « piggyback » siRNA into these organelles. By confining mobile DCL4-dependent antiviral 21nt siRNA within peroxisomes, P15 is able to shut down systemic RNAi and strongly promote PCV movement. This work describes a novel pathogenic strategy in which an organelle is used to neutralize host defensive molecules.
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-01673833
Contributor : Abes Star :  Contact
Submitted on : Thursday, January 11, 2018 - 5:17:11 PM
Last modification on : Wednesday, October 14, 2020 - 3:43:55 AM
Long-term archiving on: : Sunday, May 6, 2018 - 11:15:05 PM

File

INCARBONE_Marco_2016_ED414.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01673833, version 2

Collections

Citation

Marco Incarbone. In vivo study of the suppression of cell-autonomous and systemic RNA silencing by the Peanut clump virus protein P15. Virology. Université de Strasbourg, 2016. English. ⟨NNT : 2016STRAJ090⟩. ⟨tel-01673833v2⟩

Share

Metrics

Record views

261

Files downloads

61