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Elaboration of an artificial polybacterial biofilm as a model for endodontic disinfection procedures

Abstract : Management of infection is the key to a successful root canal treatment and development of a study model of endodontic biofilm which resemble structurally to its wild type counterpart seems crucial before any clinical application of different protocols. However, the in vitro reproduction of the root canal biofilm which consists of about 500 different bacterial species is very difficult. In laboratory MICORALIS (EA 7354) we were interested in conception of an artificial polybacterial. The bibliographical research allowed to choose S. salivarius, E. faecalis, F. nucleatum and P. gingivalis which are representatives of different groups of root canal biofilm colonizers. Following a series of periodic Scanning Electron Microscopies of samples and furthermore by help of FISH-Confocal imaging of 16S rRNA, we could prove the presence of these bacteria inside the biofilm structure and illustrate their distribution over the root canal system. In addition, it was possible also to confirm the maturation time needed to obtain the biofilm model, which is resistant enough to be used in vitro for endodontic disinfection investigation. After being characterized, we treated the model biofilm with different endodontic decontamination protocols
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Submitted on : Monday, January 1, 2018 - 1:32:06 AM
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Omid H. Muhammad. Elaboration of an artificial polybacterial biofilm as a model for endodontic disinfection procedures. Agricultural sciences. Université Nice Sophia Antipolis, 2016. English. ⟨NNT : 2016NICE4022⟩. ⟨tel-01673818⟩

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