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Étude des propriétés de surface du bactériophage MS2 et du norovirus murin au cours de différents traitements d’inactivation

Abstract : Although heat treatments or disinfections by oxidants have proven their virucidal efficiencies, mechanisms related to the loss of infectivity are not known. This statement could lead to a misinterpretation of the presence of viral genome on infection risk for humans in food matrices. This thesis aimed to study the evolution of surface properties (charge and hydrophobicity) for model viruses, bacteriophage MS2 and murine norovirus, during the heat, sodium hypochlorite and ozone inactivations. For both viruses, the existence of a critical temperature beyond which the viral particle was disrupted and released its genome was demonstrated. Simple treatment with RNase would then only detect infectious virus by molecular biology. The heat treatment also involved a transient increase in the hydrophobicity which highlighted conformational changes of the viral capsid. Sodium hypochlorite slightly modified the surface properties but oxidation phenomena occurred onto capsid since the bacteriophage MS2 charge has changed a little. These changes decreased the thermal resistance of the virus. Synergistic effects of both sodium hypochlorite and heat were observed on the inactivation of MS2 phages, the sensitivity of their genome to RNases and the increase in hydrophobicity of remaining infectious particles. Regarding gaseous ozone, we underlined its interest in the case of virucidal treatment of fragile food matrices. Therefore, this work specified the virus inactivation mechanisms and opened up new perspectives to discriminate infectious from non-infectious viruses but also to propose the exploration of new technological processes
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Submitted on : Monday, January 1, 2018 - 1:38:06 AM
Last modification on : Friday, October 23, 2020 - 5:00:37 PM
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  • HAL Id : tel-01673797, version 2


Adrien Brié. Étude des propriétés de surface du bactériophage MS2 et du norovirus murin au cours de différents traitements d’inactivation. Virologie. Université de Lorraine, 2017. Français. ⟨NNT : 2017LORR0001⟩. ⟨tel-01673797v2⟩



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