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Détermination de métalloprotéines par dilution isotopique associée à la spectrométrie de masse à plasma induit afin d'assurer la traçabilité métrologique en biochimie clinique

Abstract : This work is based on the implementation of metrological protocols to ensure traceability to the International System of Units (SI) of analysis in clinical biochemistry. The main objective is to develop and validate primary reference methods for the analysis of iron (Fe) and haemoglobin (Hb) in biological fluids. In a first part, a double isotope dilution (ID) reference method associated with ICPMS for the analysis of total Fe in serum allowing a connection of the result to SI was developed. In a second part, three approaches to ID for the analysis of total Hb in hemolysate were studied. A first method consists of mineralizing the sample and applying the ID-ICPMS by determining the Hb concentration through the Fe content. This developed and validated method is promising, however, it remains limited to samples where no other ferric species must be present. Thus, the implementation of the elementary speciation analysis using the ID with the HPLC-ICPMS coupling is necessary. Two modes of elementary speciation are evaluated: the non-specific mode and the specific mode. The results are encouraging, however, additional work, in terms of accuracy, will have to be done in order to be able to use these methods for the assignment of reference values.
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https://tel.archives-ouvertes.fr/tel-01673370
Contributor : Marie Palos <>
Submitted on : Friday, December 29, 2017 - 1:08:10 PM
Last modification on : Thursday, March 5, 2020 - 7:25:17 PM

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Marie Palos. Détermination de métalloprotéines par dilution isotopique associée à la spectrométrie de masse à plasma induit afin d'assurer la traçabilité métrologique en biochimie clinique. Chimie analytique. UPPA, 2016. Français. ⟨tel-01673370⟩

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