Skip to Main content Skip to Navigation
Theses

Utilisation des caroténoïdes naturels de Momordica cochinchinensis (gac) comme composés santé : extraction et bioactivité en fonction de l'origine et du procédé

Abstract : The aril of Momordica cochinchinensis (gac), plant from the Cucurbitaceae family, is the richest source of lycopene and β-carotene, which are a strong antioxidant and a pro-vitamin A, respectively, interesting for health-complements. First, a process of soft extraction-fractionation was developed for extracting effectively the natural carotenoids from gac without loss of their original quality. Then, the lycopene and β-carotene extracted from gac were analyzed and characterized. At least 95% of the extracts were composed of the all-trans isomer. They were not degraded during the heat-treatment mimicking formulation processing. Their thermal stereo-mutation was evaluated. Lycopene is more antioxidant, it is thus isomerized more rapidly than β-carotene at high temperature. The isomerization of carotenoids increases their antioxidant activity that was evaluated by the chemical test TEAC and through the hemolysis of red blood cells (KRL) in vitro. The lycopene and β-carotene from gac are notably more antioxidant than Trolox. By comparing with other sources of β-carotene, carotenoids extracted from gac in these soft conditions keep their antioxidant properties, even at high concentration, contrasting with extracts obtained in classical conditions that become prooxidant. From these results, the bioactivity of carotenoids is discussed from their quality and their origin that is their source and extraction process. For application, in addition to the fractionation process which is easily transferable to the industry scale, the heat-treatment used in this study is interesting for controlling products rich in functional carotenoids
Document type :
Theses
Complete list of metadatas

Cited literature [255 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01668508
Contributor : Abes Star :  Contact
Submitted on : Wednesday, December 20, 2017 - 10:00:46 AM
Last modification on : Friday, November 23, 2018 - 10:28:52 AM

File

these_A_PHAN_Thi_Hanh_2014.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01668508, version 1

Collections

Citation

Hanh Phan-Thi. Utilisation des caroténoïdes naturels de Momordica cochinchinensis (gac) comme composés santé : extraction et bioactivité en fonction de l'origine et du procédé. Ingénierie des aliments. Université de Bourgogne, 2014. Français. ⟨NNT : 2014DIJOS087⟩. ⟨tel-01668508⟩

Share

Metrics

Record views

234

Files downloads

4614