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Ingénierie tissulaire : Mise en oeuvre d’un procédé de fabrication d’une matrice oesophagienne biologique

Abstract : Decellularized matrixes (DM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical procedure of the esophagus is often complicated by stricture, but preclinical studies have shown that the use of a DM can mitigate stricture and promote a constructive outcome. Recognizing the potential benefits of DM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of DM from porcine esophagus. The developed protocol for esophageal DM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a good mechanical behavior. Stem cells remained viable when seeded upon the esophageal DM in vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.
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https://tel.archives-ouvertes.fr/tel-01665813
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  • HAL Id : tel-01665813, version 1

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Guillaume Luc. Ingénierie tissulaire : Mise en oeuvre d’un procédé de fabrication d’une matrice oesophagienne biologique. Biologie cellulaire. Université de Bordeaux, 2016. Français. ⟨NNT : 2016BORD0423⟩. ⟨tel-01665813⟩

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