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. Aluspray, . Vetoquinol, and F. ). Lure, Animals were given a subcutaneous injection of a cephalosporin antibiotic (cefazoline 006 mg/kg) and an opioid painkiller (buprenorphine 005 mg/kg) during the procedure and the day after. The group was split in two In these preliminarydata, the irradiation started 3 weeks after spacer insertion External Beam RadioTherapy (EBRT) was delivered at PRECI, an experimental veterinary radiotherapy platform (Villeneuve d'ASCQ, France.). An orthovoltage X-ray source (PANTAK, THERAPAX DXT 300, Gulmay Medical, Camberley, Surrey, UK) was used for the delivery of a 100kV low-energy photon beam. Rats were treated individually, under isoflurane anaesthesia, in a dorsal recumbency. A 5-cm circular applicator (SSD 30cm; 3-cm efficient field width) was directed, as a single field, on the bone defect over the medial aspect of each left and right hind limb. For each field, a 3-mm sheet of lead blocked the ipsilateral aspect of the pelvis, The skin was closed with Michel staples and then covered with aluminum spray Twenty-five daily fractions (5-day week) of 2 Gray were administered to the implant (skin dose of 2.2 Gy) for a total dose, p.50

. Gray, During EBRT procedure and the period between EBRT and euthanasia, rats were evaluated every day, and weighted weekly. Irradiation related complications were screened: local skin inflammation, wound complication

H. and H. Matrix, After the EBRT procedure defects were opened and spacers were removed. Cavities were filled with the beads of The defect has been closed following the same procedure than for the first step of surgery. Rats were sacrificed 12 weeks after the second surgery with a CO2 overdose. Samples were placed in a 4% (w/v) paraformaldehyde solution for 24 hours, then kept in 0.1 M PBS pH 7.4 at 4°C for µCT and histological analysis. The chronological processus of this technique is presented in Figure 53