Métabolites de Pseudotsuga menziesii : approche métabolomique et rôle dans la résistance

Abstract : For the identification and access to secondary metabolites of Douglas-fir, the optimization of the extraction conditions was carried out in different solvents. The identification of compounds by LC-MS / MS was favored. LC-ESI-MS / MS analysis of thewood extracts from the three zones was carried out to identify the different groups of metabolites present in each zone. About fifty metabolites have been identified. The most representative groups of metabolites are polyphenols and terpenes, which are said to have antioxidant, antifungal properties that are at origin of the natural durability of wood. In a second step, the relative quantification of metabolites of interest was carried out using wood samples taken during the 4 seasons of the year. The results showed that duramen was the richest metabolite area in any season. However, the amount of each metabolite varies differently depending on the season. For a majority of quantified compounds, spring and summer are the seasons in which wood accumulates a large amount of metabolites. These results confirm that the duramen is the area richest in protective metabolites. This information can be useful for selecting the most resistant lines and improving the natural durability of wood.
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Henri Mbakidi-Ngouaby. Métabolites de Pseudotsuga menziesii : approche métabolomique et rôle dans la résistance. Chimie organique. Université de Limoges, 2017. Français. ⟨NNT : 2017LIMO0056⟩. ⟨tel-01662455⟩

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