Detection of molecular changes induced by different classes of antibiotics against Escherichia coli and Vibrio parahaemolyticus using Raman and Infra-red spectroscopies

Abstract : The present study aimed to explore Raman and IR in combination with PCA to detect molecularchanges induced by different classes of antibiotics(ampicillin, cefotaxime, tetracycline and ciprofloxacin) against two bacterial models (E.coli and V. parahaemolyticus) In E. coli,ampicillin and cefotaxime treatments led to a decrease of protein bands in both Raman and IR,an increase of carbohydrates in IR. Tetracycline addition caused an increase of nucleic acids, a sharp decrease of phenylalanine in Raman, a decrease of protein bands and an increase of DNA in both Raman and IR. For ciprofloxacin, an increase of nuleic acids in Raman, an increase of protein bands and DNA in IR were observed. In V. parahaemolyticus, cefotaxime resulted in a decrease of protein bands in both Raman and IR,an increase of polysaccharides in IR. Tetracycline increase of polysaccharides and a decrease of proteins in both Raman and Infra-red were noticed. Clear discrimination of antibiotic-treated samples compared to the control was recorded for the three antibiotic classes in both E. coli and V. parahaemolyticus. For the latter, resistance pattern has also been observed for ampicillin. This work lays the foundations for an understanding of the mechanisms of antibiotic resistance in the bacterial systems.
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Ngoc Thanh Xuan Nguyen. Detection of molecular changes induced by different classes of antibiotics against Escherichia coli and Vibrio parahaemolyticus using Raman and Infra-red spectroscopies. Bacteriology. Université du Maine, 2017. English. ⟨NNT : 2017LEMA1025⟩. ⟨tel-01661578⟩

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