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Biomatériaux auto-supportés et dégradables pour l'ingénierie tissulaire : association d'un gel de fibrine et un réseau de polymère synthétique

Abstract : Tissue engineering aims to regenerate deficient tissues and organs. Fibrin gels are widely used for the reconstruction of various tissues. However, at physiological concentration, they can’t be handled. To improve their mechanical properties, they can be combined with a synthetic polymeric network (PVA or PEO) in an Interpenetrating Polymer Network (IPN) architecture. These IPN can be made biodegradable by crosslinking the Bovine Serum Albumin modified by methacrylate groups (BSAm) with the synthetic partner.Depending on the composition, these materials can be fully degraded or fragmented after several days of incubation with thermolysin, a proteolytic enzyme. The degradation kinetics of these hydrogels were studied by following the release of proteic fragments from the material and by the loss of viscoelastic properties. The biocompatibility was also verified: fibroblasts cultivated on the surface show a viability of over 90% after 5 weeks of culture and the proliferation is followed by the synthesis of Extracellular Matrix macromolecules.To add a bactericide property, and to increase their mechanical resistance, ammonium salts were incorporated in those IPN. Lastly, the synthesis of these IPN were adapted, starting from whole blood plasma. Rejection phenomena upon implantation should thus be hindered. Moreover, blood plasma contains a wide variety of growth factors and bioactive molecules, which should improve tissue regeneration.
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Submitted on : Wednesday, November 22, 2017 - 3:46:06 PM
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  • HAL Id : tel-01644802, version 1

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Marie Deneufchatel. Biomatériaux auto-supportés et dégradables pour l'ingénierie tissulaire : association d'un gel de fibrine et un réseau de polymère synthétique. Médecine humaine et pathologie. Université de Cergy Pontoise, 2016. Français. ⟨NNT : 2016CERG0877⟩. ⟨tel-01644802⟩

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