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Caractérisation de biomarqueurs cellulaires pour étudier la plasticité mammaire au cours de la lactation chez la vache laitière.

Abstract : Research of lactation function of the bovine mammary gland remains essential in dairy farming. The mammary gland is an organ composed of epithelial cells, myoepithelial cells, fibroblasts, adipose cells and endothelial cells. The identification and evolution of cell populations in the bovine mammary gland during lactation is currently unknown. The objectives of this thesis were 1) to obtain a panel of biomarkers capable of identifying different cells within the mammary gland 2) to follow the evolution of these cells throughout a lactation cycle. The cell surface biomarkers (CD49f, CD24, CD10 and EpCAM) allowed us to phenotype two bovine mammary cell lines. We also used these cell biomarkers to phenotype cell populations during lactation.For this objective mammary gland explants were obtained by biopsies taken on 5 primiparous lactating cows at four different times during lactation. After the biopsies were digested, the cells obtained were phenotyped by flow cytometry. Analysis of the results revealed a positive correlation between the CD49f+ and CD49f+/CD24- cell populations and milk yield. We also found evidence that one cell population (CD49f-EpCAM-) was increased during lactation. In general these results suggest that biomarker expression can be utilized to identify the phenotype and the evolution of different cell types during lactation in the bovine mammary gland.
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Submitted on : Tuesday, November 21, 2017 - 11:17:13 AM
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Magdalena Arevalo Turrubiarte. Caractérisation de biomarqueurs cellulaires pour étudier la plasticité mammaire au cours de la lactation chez la vache laitière.. Biologie animale. Agrocampus Ouest, 2016. Français. ⟨NNT : 2016NSARB280⟩. ⟨tel-01643218⟩

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