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Étude des facteurs de la biogenèse des ribosomes dans les progéniteurs neuraux de poisson zèbre

Abstract : While ribosome biogenesis has been consideredas an ubiquitous mechanism, steps of thisprocess have recently been shown to be tissuespecific. Zebrafish optic tectum (OT) is asuitable model to study cell proliferation sincecells at different differentiation states arespatially partitioned.During my PhD, I examined whether ribosomebiogenesis genes may have specific roles inneuroepithelial progenitor cells (NePCs).Taking advantage of a previous transcriptomicanalysis, I first screened for new candidatesaccumulated in NePCs. I decided to focus onproliferation-associated 2G4 (pa2g4/ebp1),which was expressed preferentially in NePCs.This gene promotes or represses cellproliferation in normal organisms or duringtumorigenesis. I designed a strategy for theinducible expression and cell specificexpression of this gene.Fibrillarin (Fbl), a small nucleolarmethyltransferase is also preferentiallyexpressed in NePCs. It plays an important rolein cancer. I showed that fbl mutants displayedspecific OT defects linked to a massiveapoptosis and an absence of neuraldifferentiation. I also demonstrated deficienciesin the ribosome translational activity.Additionally, fbl mutants showed impaired Sphaseprogression. Our data suggest that fbl isessential for the proliferation of zebrafishneuronal progenitors.
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https://tel.archives-ouvertes.fr/tel-01617875
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Submitted on : Tuesday, October 17, 2017 - 11:15:48 AM
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Stéphanie Bouffard. Étude des facteurs de la biogenèse des ribosomes dans les progéniteurs neuraux de poisson zèbre. Development Biology. Université Paris Saclay (COmUE), 2017. English. ⟨NNT : 2017SACLS228⟩. ⟨tel-01617875⟩

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