Recyclage membranaire : rôle de la protéine MICAL-L1 et de son partenaire PACSINE3

Abstract : The recycling to the plasma membrane of receptors and lipids is tightly regulated and is essential for PM homeostasis, adhesion and cell migration. It requires small GTPase Rab proteins and their effectors. The MICAL-L1 protein, an effector of several Rabs including Rab 8, 11, 13 and 35, has been shown to play an important role in the recycling. Here, we report a novel interaction between MICAL-L1 and the BAR domain containing protein PACSIN3/Syndapin3 that contributes to generate tubular recycling endosomes. MICAL-L1 is required for the localization of PACSIN3 to endosomal membranes. Importantly, disruption of MICAL-L1/PACSIN3 interaction promotes the transferrin receptor (TfR) delivery back to the plasma membrane. The MICAL-L1/PACSIN3 complex accumulates in elongated tubules that contain transferrin carriers. The dynamic of transferrin positive endosomes segregation from MICAL-L1/PACSIN3 tubules suggests that MICAL-L1/PACSIN3 complex controls TfR recycling endosomes delivery to the plasma membrane. Our data provide novel mechanistic insights on the dynamical regulation of the plasma membrane recycling pathway.
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Romain Sikora. Recyclage membranaire : rôle de la protéine MICAL-L1 et de son partenaire PACSINE3. Biologie cellulaire. Université Sorbonne Paris Cité, 2015. Français. ⟨NNT : 2015USPCB179⟩. ⟨tel-01590458⟩

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