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Theses

Biomateriaux collagène / gélatine : des phases cristal-liquides aux matériaux hybrides

Abstract : The object of this thesis was to study the in vitro self-assembly phenomena of native, denatured or modified collagen type I.In the first part, we analysed dense solutions of collagen I and gelatin A (in acidic medium) by polarized light microscopy and polarization resolved SHG microscopy (P-SHG). We have thus observed the first mesophase obtained from gelatin. We then studied the impact of gelatin on collagen I plywood mesophases and showed that collagen could be replaced by gelatin up to 20% without affecting the formation of the plywood phases.Collagen/gelatin matrices obtained after fibrillogenesis were characterized at different scales by using Differential Scanning Calorimetry (DSC), Transmission Electron Microscopy (TEM), P-SHG and Magnetic Resonance Imaging (MRI). We showed that these matrices have a hierarchical structure of fractal type and that gelatin stabilizes collagen.In the second part, we studied the self-assembly of collagen I modified with rifamycin SV for the synthesis of controlled delivery gels.We used different methods of assembly in order to reach our goal and we characterized the chemical, rheological and antibiotic properties of the obtained gels. To better understand the system, we studied the structure of modified collagen assemblies in a diluted systems with cryo-TEM and atomic force microscopy coupled with infrared spectroscopy (AFMIR).
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François Portier. Biomateriaux collagène / gélatine : des phases cristal-liquides aux matériaux hybrides. Matériaux. Université Pierre et Marie Curie - Paris VI, 2016. Français. ⟨NNT : 2016PA066655⟩. ⟨tel-01585249⟩

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