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Développements méthodologiques en TLC/MALDITOF MS et GC/MS pour l’analyse des composés terpénoïdes présents dans les résines végétales

Abstract : Resins are hydrocarbon secretions of many plants and well known for their protective benefits. They have been used as raw materials for a wide range of applications (pharmaceutic, cosmetic and artistic). Plant resins are complex mixtures of organic substances mainly terpenoid compounds which constitute the most abundant and structurally diverse group of plant secondary metabolites. The chemical characterization of this material results in long and difficult separation due to the wide range of polarity and volatility of its constituents. The aim of this work was to develop new analytical approaches to improve the identification of resins certifying their origin and ensuring the quality control. For that purpose two analytical methods were selected: TLC and GC approaches hyphenated to mass spectrometry. TLC-1D and TLC-2D allow a rapid screening and first visual differences of resins. The innovating TLC coupling to MALDI-TOF-MS gives a clear identification of major markers (triterpenic acids). In order to have complementary information about the composition of resins, a gas chromatography-mass spectrometry (GC-MS) method was developed to analyze volatile to non-volatile compounds. The various stages of optimization were based on experimental design and statistical (PCA and HAC) approaches. For closely related resins, a quantitative approach was investigated based on a complete validation for major markers. This work allows the development of two complementary techniques that give a powerful approach for fast and reliable differentiation of various resins even the closest ones.
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Submitted on : Monday, September 4, 2017 - 2:58:07 PM
Last modification on : Thursday, August 22, 2019 - 1:20:11 AM

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Zaïneb Jemmali. Développements méthodologiques en TLC/MALDITOF MS et GC/MS pour l’analyse des composés terpénoïdes présents dans les résines végétales. Chimie organique. Université d'Orléans, 2016. Français. ⟨NNT : 2016ORLE2061⟩. ⟨tel-01581308⟩

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