Une approche réseau pour l’inférence du rôle des microARN dans la corégulation des processus biologiques

Abstract : RNA interference is a process in which a small non-coding RNA will bind to a specific messenger RNA and regulate its expression. This evolutionary conserved mechanism is found in all superior eukaryotes from plants to mammals. Nowadays, we know that RNA interference is a major regulatory process involved in developmental biology and tumor progression. MicroRNAs (miRNAs) are endogenous (coded in and produced by the cell) non-coding RNAs which are able to regulate a whole set of genes, typically hundreds of genes. This doctoral thesis consisted in the analysis of the miRNA mediated coregulation through a network approach based on target sharing. Coregulation is the process where many different miRNAs will regulate the same set of genes and thus the same biological process. In particular, the work consisted in the inference of a miRNA network, in its topological analysis and also its biological interpretation. Indeed, the final aim of the work was to generate new biological hypothesis. As such, two different groups of miRNAs were first retrieved. One of them was predicted to be involved in the small GTPase signaling and was further validated in vitro. Moreover, a miRNA community involved in the maintenance of stem cells pluripotency was also discovered. Finally, a systemic analysis of the target-based miRNAs network was conducted to better understand their integration with biologic networks and their role in cell fate.
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  • HAL Id : tel-01562833, version 1



Ricky Bhajun. Une approche réseau pour l’inférence du rôle des microARN dans la corégulation des processus biologiques. Médecine humaine et pathologie. Université Grenoble Alpes, 2015. Français. ⟨NNT : 2015GREAS045⟩. ⟨tel-01562833⟩



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