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Impact des contraintes physiques sur la maturation des mégacaryocytes : rôle de la rigidité de l'environnement

Abstract : Megakaryopoiesis is the process of differentiation and maturation of megakaryocytes (MKs) in the aim to produce platelets able to prevent hemorrhages. These mechanisms are not well known. To better understand the process of platelet formation, we mimicked the medullar microenvironment in vitro, in 3D using hydrogel of stiffness comparable to the bone marrow. In this study we: i) characterized the physical properties of the hydrogel and design the culture of hematopoietic progenitors in this system, ii) showed the MKs ability to feel the physical constraints of their environment, then iii) showed the impact of these constraints on the MK maturation and proplatelet generation, and finally iv) highlighted the MK response to stiffness. MKs are “mecanosensitives”, being able to feel and to adapt to the physicals modifications of the environment. The activation of mechanotransduction pathways (including MKL1) and the cytoskeleton reorganization in response to extracellular physical constraints improves MK maturation, in terms of ploïdy, ultrastructure and ultimately proplatelet generation.
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Submitted on : Tuesday, July 11, 2017 - 10:43:16 AM
Last modification on : Friday, December 4, 2020 - 3:10:05 PM
Long-term archiving on: : Wednesday, January 24, 2018 - 10:12:59 PM


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  • HAL Id : tel-01560013, version 1



Alicia Aguilar. Impact des contraintes physiques sur la maturation des mégacaryocytes : rôle de la rigidité de l'environnement. Embryologie et organogenèse. Université de Strasbourg, 2017. Français. ⟨NNT : 2017STRAJ014⟩. ⟨tel-01560013⟩



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