Approche métabolomique pour une caractérisation plus fine d'extraits de plantes d'intérêts pour la santé humaine

Abstract : Tinctures defined as hydro-alcoholic extracts have been used from centuries in homeopathy and phytotherapy, but their chemical compositions remain still unknown. During my PhD, metabolomics analyses of nineteen tinctures and one leader product, L52, made by Laboratoires Lehning, were conducted using untargeted metabolomic approach. We build GC-MS and UPLC-MS/MS databases to identify a large amount of metabolites. Then, we used semi-preparative HPLC with both UV and mass detection to isolate some compounds from tinctures. We used UPLC-HRMS to obtain chemical formula, a prerequisite for metabolites identification. Finally, we identified a broad range of different metabolites in each tincture, highlighting the metabolic complexity of the TMs. These molecules can now be used for quality-control and valued for a better understanding of these products on human health.
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Julien Delecolle. Approche métabolomique pour une caractérisation plus fine d'extraits de plantes d'intérêts pour la santé humaine. Biologie végétale. Université de Strasbourg, 2017. Français. ⟨NNT : 2017STRAJ012⟩. ⟨tel-01559676⟩

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